The Arsenal forward has netted 12 goals in 44 caps for England.
The national side have included the Arsenal winger for upcoming games versus Latvia and Wales, yet there's no spot for Jude Bellingham, Phil Foden or Grealish.
Gunners attacker Bukayo Saka was absent from last month's qualifier victories against Andorra and Serbia due to a hamstring injury.
Saka made a comeback for his club and found the net in their victory against the Greek side midweek.
Saka comes in for club colleague Noni Madueke, who is out of action till next month because of a knee problem.
The Three Lions meet the Welsh in a warm-up match at the national stadium on Thursday before a qualifying match in Riga on October 14.
If England win in Latvia, they will need a maximum of two points from their final two qualifiers against Serbia and Albania in the next month to reach next summer's tournament in North America.
Leverkusen's Jarell Quansah comes in for Newcastle's Tino Livramento, who is sidelined for two months after sustaining a knee issue against the Gunners recently.
Elliot Anderson and Spence, who received their maiden national team selections last month, keep their spots. Loftus-Cheek also keeps his spot, even with the recovery of Adam Wharton.
The attacker has only played one game for the manager since he became England manager, because of injury concerns.
Bellingham missed the previous squad as he was rehabilitating after a shoulder operation and has only made one start for Real Madrid after coming back to fitness.
The Manchester City player was left out from the coach’s last England squad after a loss of form and has been unable to force his way back, despite a impressive beginning to the season with Manchester City.
Grealish also misses out despite a revival in form with Everton. The 30-year-old has not played for the national team since October 2024.
Goalkeepers: Henderson (Palace), Pickford (Everton), Trafford (City)
Backline: Burn (Newcastle United), Guehi (Palace), James (the Blues), Konsa (Aston Villa), Lewis-Skelly (Arsenal), Jarell Quansah (Leverkusen), Spence (Tottenham Hotspur), Stones (City)
Midfielders: Anderson (Forest), Morgan Gibbs-White (Nottingham Forest), Jordan Henderson (the Bees), Ruben Loftus-Cheek (AC Milan), Rice (the Gunners), Rogers (Villa)
Forwards: Jarrod Bowen (West Ham United), Eberechi Eze (the Gunners), Gordon (Newcastle United), Harry Kane (Bayern), Rashford (Barcelona), Bukayo Saka (the Gunners), Ollie Watkins (Aston Villa)
Thomas Tuchel's new selection draws attention on those the boss has omitted rather than the players he has selected.
Jude Bellingham's exclusion may raise some eyebrows but Tuchel believes there is no need to recall the Madrid midfielder.
Manchester City's Phil Foden and on-loan Everton forward Grealish have both performed well at the start of the season.
The players Tuchel utilised in the team’s World Cup qualifier wins against Serbia and Andorra merit the chance to continue.
There will be regret that Arsenal's Madueke is not involved due to injury.
The Forest player has settled well at England senior level.
Tuchel is a fan of Grealish, in particular, as well as the City star.
If there is one surprise, it is the absence of Palace’s Wharton.
The star, naturally, will be required in the future.
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